3I71
Ethanolamine Utilization Microcompartment Shell Subunit, EutK C-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9717 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 65.345, 65.345, 146.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.810 - 2.100 |
| R-factor | 0.207 |
| Rwork | 0.206 |
| R-free | 0.23000 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.435 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.070 | 0.045 | 0.326 |
| Number of reflections | 11489 | ||
| <I/σ(I)> | 13.4 | ||
| Completeness [%] | 99.9 | 99.3 | 100 |
| Redundancy | 19.7 | 17.4 | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 298 | 0.1M sodium citrate, 20.4% PEG4000, 16% isopropanol, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
