3I6L
Newly identified epitope N1 derived from SARS-CoV N protein complexed with HLA-A*2402
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-06-12 |
Detector | RIGAKU RAXIS VII |
Wavelength(s) | 1.54178 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.422, 85.823, 92.221 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.940 - 2.400 |
R-factor | 0.21669 |
Rwork | 0.213 |
R-free | 0.28400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bck |
RMSD bond length | 0.013 |
RMSD bond angle | 1.443 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.940 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.099 | 0.387 |
Total number of observations | 149410 | |
Number of reflections | 22272 | |
<I/σ(I)> | 40.671 | 6.211 |
Completeness [%] | 98.6 | 97.5 |
Redundancy | 6.7 | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.9 | 277 | 0.2M Ammonium sulfate, 0.1M Tris pH 8.9, 25% w/v Polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |