3I6E
CRYSTAL STRUCTURE OF MUCONATE LACTONIZING ENZYME FROM Ruegeria pomeroyi.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-12 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 109.135, 135.358, 113.024 |
| Unit cell angles | 90.00, 103.18, 90.00 |
Refinement procedure
| Resolution | 24.870 - 1.700 |
| R-factor | 0.235 |
| Rwork | 0.235 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.084 |
| Number of reflections | 342184 |
| Completeness [%] | 97.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% peg 3350, 0.1M bis-Tris, 0.2M sodium chloride, , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
