3I5X
Structure of Mss116p bound to ssRNA and AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-16 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.91842 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.540, 126.524, 55.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.900 |
| R-factor | 0.19229 |
| Rwork | 0.191 |
| R-free | 0.22480 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.149 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0070) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.077 | 0.389 |
| Number of reflections | 48438 | |
| <I/σ(I)> | 30.6 | 4.9 |
| Completeness [%] | 96.4 | 82.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 0.2 M succinate, 15% PEG 3350 , pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
