3I59
Crystal structure of MtbCRP in complex with N6-cAMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97910 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.732, 75.716, 63.639 |
| Unit cell angles | 90.00, 110.91, 90.00 |
Refinement procedure
| Resolution | 19.477 - 2.290 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3i54 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.802 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.290 | 2.290 |
| Rmerge | 0.058 | 0.419 |
| Number of reflections | 22400 | |
| <I/σ(I)> | 15.1 | 2.6 |
| Completeness [%] | 97.9 | 89 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 2 microliters of protein + 2 microliters of well solution. Well solution was 0.4M NaH2PO4/1.6M K2HPO4, 0.1M Imidazole pH 8.0, 0.2M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
