3I57
Type 2 repeat of the mucus binding protein MUB from Lactobacillus reuteri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2008-10-08 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 1.033 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.861, 45.846, 191.573 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.620 - 1.800 |
R-factor | 0.206 |
Rwork | 0.204 |
R-free | 0.25800 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.351 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 40.620 |
High resolution limit [Å] | 1.800 |
Number of reflections | 36176 |
Completeness [%] | 96.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.2 M ammonium formate, 22 % (w/v) PEG 3350, unbuffered, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |