3I4H
Crystal structure of Cas6 in Pyrococcus furiosus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 18-ID |
Synchrotron site | APS |
Beamline | 18-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-16 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.5418,1.5621,1.7321 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 84.745, 84.745, 81.679 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.500 - 2.250 |
R-factor | 0.22951 |
Rwork | 0.226 |
R-free | 0.28896 |
Structure solution method | SAD |
RMSD bond length | 0.033 |
RMSD bond angle | 2.429 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.500 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
<I/σ(I)> | 2.4 | 2.4 |
Completeness [%] | 89.0 | 48 |
Redundancy | 17.7 | 17.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 303 | 0.02 M Magnesium chloride hexahydrate, 0.05 M MES monohydrate pH 6.0, 15% v/v 2-Propanol, temperature 303K, VAPOR DIFFUSION, HANGING DROP |