3I40

Human insulin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-10
Detector	MAR CCD 165 mm
Spacegroup name	I 21 3
Unit cell lengths	77.220, 77.220, 77.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.525 - 1.850
R-factor	0.2016
Rwork	0.200
R-free	0.22760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g4m
RMSD bond length	0.005
RMSD bond angle	0.760
Data reduction software	DENZO
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	54.636
High resolution limit [Å]	1.850
Number of reflections	6677

Crystallization Conditions

crystal ID	method	pH	temperature	details
1