3I32
Dimeric structure of a Hera helicase fragment including the C-terminal RecA domain, the dimerization domain, and the RNA binding domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-03-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 64.960, 64.960, 153.564 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.420 - 2.800 |
| R-factor | 0.2553 |
| Rwork | 0.253 |
| R-free | 0.29250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Hera RBD and 2eaq |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.155 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.420 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.081 | 0.911 |
| Number of reflections | 12078 | |
| <I/σ(I)> | 28.2 | 1.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 50 | 51.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 0.1 M MES-NaOH pH 6.5, 15-18% ethylene glycol, 0.3-0.5 M NaCl, 3-6% PEG 20 000, VAPOR DIFFUSION, temperature 277K |
