3I31
Hera helicase RNA binding domain is an RRM fold
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2009-06-05 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 37.516, 37.516, 136.890 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.222 - 1.800 |
| R-factor | 0.2015 |
| Rwork | 0.200 |
| R-free | 0.22350 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.202 |
| Data reduction software | XDS |
| Data scaling software | SADABS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.180 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 9812 | |
| <I/σ(I)> | 7.5 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.4 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M NaCac pH 6.5, 16-18% P8000, 0.3-0.4M Zn(OAc)2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.1M NaCac pH 6.5, 16-18% P8000, 0.3-0.4M Zn(OAc)2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
