3I2M
The Crystal Structure of PF-8, the DNA Polymerase Accessory Subunit from Kaposi s Sarcoma-Associated Herpesvirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Detector technology | CCD |
| Collection date | 2008-01-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | .97918 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 58.300, 58.300, 386.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.150 - 2.810 |
| R-factor | 0.25311 |
| Rwork | 0.252 |
| R-free | 0.28131 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.839 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.5.0093) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.095 | 0.492 |
| Number of reflections | 10103 | |
| <I/σ(I)> | 18.012 | 2.7 |
| Completeness [%] | 95.2 | 62.1 |
| Redundancy | 11.1 | 0.049 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 295 | 18% PEG 3350, 100 mM Tris HCl pH 8.2, 0.2 M lithium chloride, and 20 mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
