3I2D
Crystal Structure of S. Cerevisiae SUMO E3 Ligase SIZ1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 44.487, 88.980, 105.598 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.020 - 2.600 |
Rwork | 0.211 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.500 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.020 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.073 | 0.434 |
Number of reflections | 13595 | |
<I/σ(I)> | 22 | 2.6 |
Completeness [%] | 97.8 | 89.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 288 | 3.5 M NaCl, 100 mM HEPES 7.5 and either 5% glycerol or xylitol, VAPOR DIFFUSION, HANGING DROP, temperature 288K |