3HZP

Crystal structure of NTF2-like protein of unknown function MN2A_0505 from Prochlorococcus marinus (YP_291699.1) from Prochlorococcus sp. NATL2A at 1.40 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-18
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91162,0.97929,0.97918
Spacegroup name	P 32 2 1
Unit cell lengths	58.046, 58.046, 63.620
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.025 - 1.400
R-factor	0.168
Rwork	0.167
R-free	0.18400
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.527
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.5)
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.025	29.020	1.440
High resolution limit [Å]	1.400	6.260	1.400
Rmerge	0.075	0.045	0.748
Total number of observations		1473	9847
Number of reflections	24917
<I/σ(I)>	6.295	14.2	1
Completeness [%]	100.0	98.5	100
Redundancy	5.4	4.6	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	277	40.0000% PEG-600, 0.1M Citrate pH 5.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K