3HZH
Crystal structure of the CheX-CheY-BeF3-Mg+2 complex from Borrelia burgdorferi
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2008-02-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 63.705, 63.705, 175.685 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.640 - 1.960 |
| Rwork | 0.248 |
| R-free | 0.25900 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.021 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | RESOLVE (ShelxD) |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.640 | 1.980 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Number of reflections | 45929 | |
| <I/σ(I)> | 26 | 3.1 |
| Completeness [%] | 93.6 | 96 |
| Redundancy | 6 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | Ammonium Sulfate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
