3HYT
Structural Basis of GDP Release and Gating in G Protein Coupled Fe2+ Transport
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.000, 46.410, 120.070 |
| Unit cell angles | 90.00, 94.95, 90.00 |
Refinement procedure
| Resolution | 45.080 - 2.740 |
| R-factor | 0.23045 |
| Rwork | 0.227 |
| R-free | 0.29398 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.000 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Completeness [%] | 96.7 | 82 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 30% PEG, 100mM MgCl2, 1mM mant-GMPPNP, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
