3HY8
Crystal Structure of Human Pyridoxine 5'-Phosphate Oxidase R229W Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-23 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 82.270, 82.270, 58.788 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 22.020 - 2.500 |
| R-factor | 0.229 |
| Rwork | 0.226 |
| R-free | 0.28700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1nrg |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.630 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.056 | 0.330 |
| Total number of observations | 8447 | |
| Number of reflections | 8215 | |
| <I/σ(I)> | 22.5 | 6.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.66 | 9.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 295 | mono-ammonium dihydrogen phosphate, Sodium Citrate, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
