3HXQ
Crystal Structure of Von Willebrand Factor (VWF) A1 Domain in Complex with DNA Aptamer ARC1172, an Inhibitor of VWF-Platelet Binding
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-17 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 117.781, 48.512, 62.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.000 - 2.694 |
| R-factor | 0.223 |
| Rwork | 0.221 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hxo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.725 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.000 | 2.800 |
| High resolution limit [Å] | 2.694 | 2.690 |
| Rmerge | 0.080 | 0.316 |
| Number of reflections | 9819 | |
| <I/σ(I)> | 17.2 | 2.8 |
| Completeness [%] | 94.8 | 83.9 |
| Redundancy | 6.4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 293 | 0.2 M NH4Ac, 0.1 M NaAc, 30% PEG4000, pH 4.6, EVAPORATION, temperature 293K |
