3HX4
Crystal structure of CDPK1 of Toxoplasma gondii, TGME49_101440, in presence of calcium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-05-27 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.236, 95.605, 101.612 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.950 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.194 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.063 | 0.997 |
| Number of reflections | 41316 | |
| <I/σ(I)> | 31.262 | 1.23 |
| Completeness [%] | 99.0 | 90.8 |
| Redundancy | 5.5 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 25 % PEG 3350, 0.2 M LiSO4, Tris-HCl pH 8.0, 2 mM AMPPNP, 2 mM CaCl2, 4 mM MgCl2, 6.25 mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
