3HX1
Crystal structure of the Slr1951 protein from Synechocystis sp. Northeast Structural Genomics Consortium Target SgR167A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97931, 0.97907, 0.91162 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 56.130, 56.130, 290.868 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.300 - 2.500 |
| Rwork | 0.232 |
| R-free | 0.24400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXDE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.141 | 0.629 |
| Number of reflections | 17793 | |
| <I/σ(I)> | 21.8 | 2.3 |
| Completeness [%] | 99.4 | 99.6 |
| Redundancy | 9.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microbatch under oil | 8 | 291 | 40 % PEG 1000, 0.1M potassium phosphate monobasic, 0.1M TrisHCl, pH 8.0, Microbatch under oil, temperature 291K |
