3HWS
Crystal structure of nucleotide-bound hexameric ClpX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-10-27 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.085, 178.498, 201.369 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.081 - 3.250 |
| R-factor | 0.245 |
| Rwork | 0.243 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.687 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.370 |
| High resolution limit [Å] | 3.250 | 7.000 | 3.250 |
| Rmerge | 0.061 | 0.037 | 0.571 |
| Number of reflections | 32170 | ||
| <I/σ(I)> | 23.694 | ||
| Completeness [%] | 99.5 | 98 | 100 |
| Redundancy | 5.9 | 5.5 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | Sodium acetate, ammonium sulfate Soaked overnight in mother liquor plus 5 mM ATP-G-S / 5 mM magnesium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
