3HWJ
Crystal structure of the second PHR domain of Mouse Myc-binding protein 2 (MYCBP-2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-13 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.877, 99.922, 83.054 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.940 - 2.250 |
R-factor | 0.201 |
Rwork | 0.198 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Poly ala based on the PDB Code 3GBW |
RMSD bond length | 0.017 |
RMSD bond angle | 1.715 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.800 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 18914 | |
<I/σ(I)> | 8.7 | 3.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.3 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 294 | 2000mM DL Malic acid, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |