3HW6
Crystal structure of avian influenza virus PA_N in complex with Mn
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Detector technology | CCD |
| Collection date | 2009-03-26 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 51.504, 60.211, 67.215 |
| Unit cell angles | 96.06, 96.49, 109.66 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.24283 |
| Rwork | 0.240 |
| R-free | 0.28870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ebj |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.818 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.303 |
| Number of reflections | 19014 | |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 17.5% PEG3350(w/v), 100mM MgAc, 100mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
