3HVT

STRUCTURAL BASIS OF ASYMMETRY IN THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 REVERSE TRANSCRIPTASE HETERODIMER

Replaces:  2HVTReplaces:  1HVT

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	223.600, 69.900, 105.500
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.266
Rwork	0.266
RMSD bond length	0.016
RMSD bond angle	2.500
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.900 *
Rmerge	0.090 *
Number of reflections	33169 *
Completeness [%]	1.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RT	6-8 (mg/ml)
10	1	reservoir	beta-octylglucoside	0.2 (%(w/v))
11	1	reservoir	glycerol	10 (%(v/v))
12	1	reservoir	PEG8000	14 (%(w/v))
13	1	reservoir	sodium azide	0.02 (%)
2	1	drop	Bis-Tris propane	25 (mM)
3	1	drop		50 (mM)
4	1	drop	beta-octylglucoside	0.1 (%(w/v))
5	1	drop	glycerol	5 (%(v/v))
6	1	drop	PEG8000	7 (%(w/v))
7	1	drop	sodium azide	0.01 (%)
8	1	reservoir	Bis-Tris propane	50 (mM)
9	1	reservoir		100 (mM)