3HVN

Crystal structure of cytotoxin protein suilysin from Streptococcus suis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	170
Detector technology	CCD
Collection date	2008-10-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00000
Spacegroup name	P 31 2 1
Unit cell lengths	95.720, 95.720, 117.046
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.409 - 2.852
R-factor	0.28
Rwork	0.279
R-free	0.29400
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.475
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.950
High resolution limit [Å]	2.850	6.140	2.850
Rmerge	0.052	0.043	0.316
Number of reflections	13204
<I/σ(I)>	30.364
Completeness [%]	88.5	85.5	66.4
Redundancy	5.7	5.6	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7	289	8% polyethylene glycol (PEG)3350, 0.12M sodium citrate (pH7.0), 5% 1,2,3-heptanetriol isomer H, 2% 1,1,1,3,3,3-hexafluoro-2-propanol, temperature 289K
2