3HU6

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx/BTB/3-box-PucSBC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Collection date	2008-07-31
Spacegroup name	P 21 21 21
Unit cell lengths	63.740, 107.740, 130.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.700
Rwork	0.240
R-free	0.29640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 3HQH 3htm
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	25440
<I/σ(I)>	20.1	2.4
Redundancy	9.4	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1