3HTC

THE STRUCTURE OF A COMPLEX OF RECOMBINANT HIRUDIN AND HUMAN ALPHA-THROMBIN

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	90.540, 90.540, 132.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.300
R-factor	0.193 *
Rwork	0.193
RMSD bond length	0.013
RMSD bond angle	2.400
Refinement software	EREF

Data quality characteristics

	Overall
High resolution limit [Å]	2.300 *
Rmerge	0.042 *
Total number of observations	164250 *
Number of reflections	23521 *
Completeness [%]	91.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	28-30 (%)
2	1	reservoir		0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir		1 (mM)