3HSV
Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx-MacroH2ASBCpep2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 88.953, 43.086, 87.453 |
| Unit cell angles | 90.00, 118.15, 90.00 |
Refinement procedure
| Resolution | 31.230 - 1.430 |
| R-factor | 0.17528 |
| Rwork | 0.174 |
| R-free | 0.20576 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cr2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.226 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.480 |
| High resolution limit [Å] | 1.430 | 1.430 |
| Number of reflections | 54191 | |
| <I/σ(I)> | 31.7 | 14.8 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
