3HSN
Ternary structure of neuronal nitric oxide synthase with NHA and CO bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL1-5 |
| Synchrotron site | SSRL |
| Beamline | BL1-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-23 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.160, 110.847, 165.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.090 - 1.910 |
| R-factor | 0.169 |
| Rwork | 0.167 |
| R-free | 0.21000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1lzx |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.380 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.064 | 0.496 |
| Number of reflections | 73350 | |
| <I/σ(I)> | 12.7 | 2.1 |
| Completeness [%] | 97.7 | 91.8 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.8 | 278 | PEG3350, MES, ammonium acetate SDS, GSH, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
