3HSM
Crystal structure of distal N-terminal beta-trefoil domain of Ryanodine Receptor type 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-08 |
| Detector | SBC-3 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 140.597, 35.299, 78.940 |
| Unit cell angles | 90.00, 99.37, 90.00 |
Refinement procedure
| Resolution | 30.870 - 2.500 |
| Rwork | 0.229 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xzz |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.251 |
| Number of reflections | 13915 | |
| <I/σ(I)> | 14.938 | |
| Completeness [%] | 98.4 | 94.4 |
| Redundancy | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 0.1M MES, 0.1M MgCl2, 24% PEG 3350, 5mM DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
