3HQX
Crystal structure of protein of unknown function (DUF1255,PF06865) from Acinetobacter sp. ADP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-14 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 65.050, 25.952, 55.230 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.500 - 1.660 |
R-factor | 0.16471 |
Rwork | 0.163 |
R-free | 0.20150 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.596 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.500 | 1.720 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.040 | 0.115 |
Number of reflections | 11471 | |
<I/σ(I)> | 51 | 13 |
Completeness [%] | 98.4 | 95.3 |
Redundancy | 4.6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 25% Peg 3350 .2 M Lithium sulfate 0.1 M Hepes pH7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |