3HQM
Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx-CiSBC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Collection date | 2008-06-12 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.281, 48.100, 49.860 |
| Unit cell angles | 63.00, 64.04, 62.89 |
Refinement procedure
| Resolution | 25.570 - 1.740 |
| R-factor | 0.17928 |
| Rwork | 0.177 |
| R-free | 0.21777 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hqh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.192 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 31963 | |
| <I/σ(I)> | 30.3 | 9.5 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
