3HQG

Crystal structure of restriction endonuclease EcoRII catalytic C-terminal domain in complex with cognate DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	100
Detector technology	CCD
Collection date	2006-11-13
Detector	MAR CCD 165 mm
Wavelength(s)	0.8080
Spacegroup name	P 21 21 2
Unit cell lengths	77.054, 57.971, 61.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.840 - 2.600
R-factor	0.242
Rwork	0.236
R-free	0.29300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1na6
RMSD bond length	0.006
RMSD bond angle	1.012
Data reduction software	MOSFLM
Data scaling software	SCALA (3.2.19)
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.836	47.840	2.740
High resolution limit [Å]	2.110	8.220	2.600
Rmerge	0.047	0.028	0.330
Total number of observations		3114	14992
Number of reflections	8835
<I/σ(I)>	12.41	18.4	2.3
Completeness [%]	99.8	98	99.8
Redundancy	11.7	9.7	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	26% PEG1500, 25% glycerol, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K