3HQB
Crystal structure of human desarg-C5A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991-06-19 |
| Detector | SIEMENS-NICOLET X100 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 54.720, 54.720, 96.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.799 - 3.299 |
| R-factor | 0.2191 |
| Rwork | 0.210 |
| R-free | 0.30690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hqa |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.516 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.300 |
| High resolution limit [Å] | 3.299 |
| Number of reflections | 2707 |
| Completeness [%] | 98.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 298 | 2.4 M sodium chloride, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
