3HPN
Ligand recognition by A-class EPH receptors: crystal structures of the EPHA2 ligand-binding domain and the EPHA2/EPHRIN-A1 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-08-02 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.271, 91.187, 91.132 |
| Unit cell angles | 117.14, 97.27, 100.79 |
Refinement procedure
| Resolution | 30.000 - 2.520 |
| R-factor | 0.2362 |
| Rwork | 0.232 |
| R-free | 0.30740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3etp |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.492 |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.269 | |
| Number of reflections | 36900 | |
| <I/σ(I)> | 9.2 | 3.2 |
| Completeness [%] | 96.8 | 80.2 |
| Redundancy | 5.6 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 25% PEG 4,000, 100 mM Tris, 100 mM Sodium Acetate, 3% ethylene glycol, pH 8.5, vapor diffusion, hanging drop, temperature 293K |
