3HOU

Complete RNA polymerase II elongation complex I with a T-U mismatch

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-17
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9189
Spacegroup name	C 1 2 1
Unit cell lengths	394.264, 221.614, 283.446
Unit cell angles	90.00, 90.90, 90.00

Refinement procedure

Resolution	40.000 - 3.200
Rwork	0.233
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y1w
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	3.200
Number of reflections	372166
<I/σ(I)>	20.7
Completeness [%]	95.6
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	200mM ammonium acetate, 300mM SODIUM ACETATE, 50mM Hepes pH 7.0, 4-6% PEG 6000, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K