3HOU
Complete RNA polymerase II elongation complex I with a T-U mismatch
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-17 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9189 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 394.264, 221.614, 283.446 |
| Unit cell angles | 90.00, 90.90, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.200 |
| Rwork | 0.233 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y1w |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 372166 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 95.6 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 200mM ammonium acetate, 300mM SODIUM ACETATE, 50mM Hepes pH 7.0, 4-6% PEG 6000, 5mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
