3HOS
Crystal structure of the mariner Mos1 paired end complex with Mg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-09-02 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9535 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 121.232, 85.033, 131.273 |
| Unit cell angles | 90.00, 98.93, 90.00 |
Refinement procedure
| Resolution | 29.870 - 3.500 |
| R-factor | 0.222 |
| Rwork | 0.219 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2f7t |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.997 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.700 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.170 | 0.665 |
| Number of reflections | 33149 | |
| <I/σ(I)> | 9.5 | 2.5 |
| Completeness [%] | 98.8 | 99 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
