3HNK
Crystal structure of the dimeric assembly of the cyt cb562 variant RIDC-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | SIEMENS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-10-30 |
Detector | APEX II CCD |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.318, 55.484, 72.367 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.750 - 2.100 |
R-factor | 0.205 |
Rwork | 0.201 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.994 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.860 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.078 | 0.249 |
Number of reflections | 12085 | |
<I/σ(I)> | 13.4 | 4.1 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 3.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 26% PEG 2000, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |