3HN8
Crystal structure of synaptotagmin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2000-01-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97780 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 205.675, 205.675, 143.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.816 - 3.500 |
| R-factor | 0.24 |
| Rwork | 0.238 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.937 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.630 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.167 | 0.571 |
| Number of reflections | 39136 | |
| <I/σ(I)> | 10.361 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 5.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 277 | 17% MPD, 50mM CaCl2, 100mM NaCl, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
