3HMN
Crystal structure of human Mps1 catalytic domain in complex with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 94 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-13 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 70.980, 104.950, 111.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.360 - 2.700 |
| R-factor | 0.22288 |
| Rwork | 0.221 |
| R-free | 0.26457 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zmd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.973 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.360 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.072 | 0.519 |
| Number of reflections | 11436 | |
| <I/σ(I)> | 21.2 | 3 |
| Completeness [%] | 97.8 | 97 |
| Redundancy | 5.7 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 20 % PEG 300, 0.1 M HEPES, 2.5 % Isopropanol, 5 % Glycerol, 50 mM Ammonium sulphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
