3HMK
Crystal Structure of Serine Racemase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-09-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.261, 102.960, 120.923 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.100 - 2.100 |
| R-factor | 0.24389 |
| Rwork | 0.241 |
| R-free | 0.28949 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fkp |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.993 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.100 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.069 | 0.195 |
| Number of reflections | 32657 | |
| <I/σ(I)> | 10.1 | 3.5 |
| Completeness [%] | 95.8 | 74.8 |
| Redundancy | 3.17 | 1.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
