3HM8
Crystal structure of the C-terminal Hexokinase domain of human HK3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-20 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 41 |
Unit cell lengths | 83.904, 83.904, 323.327 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.800 |
R-factor | 0.245 |
Rwork | 0.245 |
R-free | 0.27000 |
Structure solution method | MR |
Starting model (for MR) | 2nzt chain A residues 468 through 913 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.054 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.666 | |
Number of reflections | 53686 | |
Completeness [%] | 98.4 | 86.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 291 | reservoir solution: 16% PEG 3350, 0.20 M Ammonium Citrate, 0.1 M HEPES. 0.005 M glucose-6-phosphate, 0.005 M ADP-Mg, 0.02 M TCEP, 1:100 (w/w) trypsin was added to the protein stock solution., pH 7.2, vapor diffusion, sitting drop, temperature 291K |