3HM6

Crystal structure of the cytoplasmic domain of human plexin B1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Detector technology	CCD
Collection date	2007-09-22
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9999
Spacegroup name	P 31 2 1
Unit cell lengths	74.346, 74.346, 214.398
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.083 - 2.402
R-factor	0.228
Rwork	0.227
R-free	0.26200
Structure solution method	SAD
Starting model (for MR)	For phasing native crystals were incubated over-night in an artificial mother liquor containing 0.001 M thimerosal.
RMSD bond length	0.013
RMSD bond angle	1.213
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.490
High resolution limit [Å]	2.400	5.160	2.400
Rmerge	0.045	0.019	0.577
Number of reflections	27657
<I/σ(I)>	15
Completeness [%]	99.9	99.2	99.9
Redundancy	9.8	9.7	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	16% peg-2000 mme, 0.1M TRIS, 0.2M TMAO, pH 8, vapor diffusion, hanging drop, temperature 291K