3HLT
The crystal structure of human haloacid dehalogenase-like hydrolase domain containing 2 (HDHD2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97240 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 68.940, 77.680, 103.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.360 - 2.300 |
| R-factor | 0.21598 |
| Rwork | 0.213 |
| R-free | 0.26580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ho4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.422 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0089) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.360 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.063 | 0.804 |
| Number of reflections | 25410 | |
| <I/σ(I)> | 10.4 | 1.5 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2.6 M Ammonium sulphate, 1% MPD, 0.1M HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
