3HKE
Tubulin-T138067: RB3 stathmin-like domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 65 |
| Unit cell lengths | 326.710, 326.710, 54.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 3.600 |
| R-factor | 0.20934 |
| Rwork | 0.207 |
| R-free | 0.25254 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sa0 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.626 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Number of reflections | 38708 | |
| <I/σ(I)> | 14.2 | 2.29 |
| Completeness [%] | 98.6 | 88.5 |
| Redundancy | 4.88 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG, PIPES BUFFER, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
