3HJT
Structure of laminin binding protein (Lmb) of Streptococcus agalactiae A bifunctional protein with adhesin and metal transporting activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-10-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.641, 70.411, 74.971 |
| Unit cell angles | 90.00, 96.14, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3cx3 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | AUTOMAR |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.054 | 0.274 |
| Number of reflections | 19066 | |
| <I/σ(I)> | 5.6 | 1.4 |
| Completeness [%] | 98.7 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5 | 293 | 30-35% (w/v) 30% PEG 2000 monoethyl ether, 0.1mM sodium citrate pH5.0, 5mM NiCl2 and 10% ethylene glycol, VAPOR DIFFUSION, temperature 293K |
