3HJB
1.5 Angstrom Crystal Structure of Glucose-6-phosphate Isomerase from Vibrio cholerae.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 125.661, 75.094, 127.456 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 29.880 - 1.500 |
| R-factor | 0.12774 |
| Rwork | 0.127 |
| R-free | 0.15070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gzd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.370 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0044) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.079 | 0.335 |
| Number of reflections | 370201 | |
| <I/σ(I)> | 13.2 | 3.4 |
| Completeness [%] | 97.7 | 85 |
| Redundancy | 3.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | Protein solution: 7.7 mg/mL protein, 0.01M Tris-HCl, 0.25M Sodium Cloride, Screen solution (PACT II, drop B11): 0.2M Calcium chloride, 0.1M MES pH 6.0, 25% w/v PEG 6000., VAPOR DIFFUSION, SITTING DROP, temperature 295K |
