3HJ1
Minor Editosome-Associated TUTase 1 with bound UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-14 |
| Detector | MARMOSAIC 325 mm CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.873, 101.999, 66.124 |
| Unit cell angles | 90.00, 111.65, 90.00 |
Refinement procedure
| Resolution | 31.050 - 1.950 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.401 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.127 | 0.490 |
| Number of reflections | 54366 | |
| <I/σ(I)> | 12.528 | |
| Completeness [%] | 96.4 | 83.5 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 291 | 0.2M lithium acetate, 25% PEG 3350, 0.5mM UTP, pH 8.0, vapor diffusion, temperature 291K |
