3HIY
Minor Editosome-Associated TUTase 1 with bound UTP and Mg
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-04 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837, 0.97876, 0.97935 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.093, 103.024, 63.041 |
Unit cell angles | 90.00, 111.01, 90.00 |
Refinement procedure
Resolution | 33.710 - 2.300 |
R-factor | 0.2 |
Rwork | 0.198 |
R-free | 0.24800 |
Structure solution method | MAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.493 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE (2.13) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.950 | 2.300 |
Rmerge | 0.092 | 0.045 | 0.480 |
Number of reflections | 67571 | ||
<I/σ(I)> | 21.66 | ||
Completeness [%] | 99.6 | 99.7 | 98.7 |
Redundancy | 3.8 | 3.9 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 291 | 0.2M lithium acetate, 25% PEG 3350, 0.5mM UTP, 10mM MgCl2, pH 8.0, vapor diffusion, temperature 291K |