3HIH
Structure of human Plk1-PBD with glycerol and sulfate in the phophopeptide binding site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-08-09 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.300, 102.300, 68.500 |
| Unit cell angles | 90.00, 93.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.551 |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.259 |
| Number of reflections | 49465 | |
| <I/σ(I)> | 23.1 | 2.9 |
| Completeness [%] | 98.4 | 85.8 |
| Redundancy | 4.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.2 M lithium sulfate monohydrate, 0.1 M bis-tris, 25% w/v PEG 3350, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
