3HIB
Crystal structure of the second Sec63 domain of yeast Brr2
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-04 |
| Detector | NOIR-1 |
| Wavelength(s) | 0.9790, 0.9789, 0.9640 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.153, 75.678, 83.955 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.674 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.084 | 0.414 |
| Number of reflections | 45853 | |
| <I/σ(I)> | 10.7 | 3.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.2 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M Sodium citrate, 17% PEG 8000, 0.2M NaCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
